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Advance Your Research with Hands-On Stem Cell Workshops

The STEMTECH Center offers immersive, hands-on training programs designed to give researchers and industry professionals the skills to excel in cutting-edge stem cell and primary cell research. Our workshops combine expert instruction with practical laboratory experience, enabling participants to confidently implement advanced techniques in their own projects.

Our Featured Workshops:

• Human iPSC Culture Workshop – Master the culture, expansion, and maintenance of human induced pluripotent stem cells.
• Neural Differentiation Workshop: PSCs → NPCs → Neurons – Learn stepwise protocols for deriving neural progenitors and mature neurons.
• Culturing Primary Cells from Human & Animal Tissues – Gain expertise in isolating and maintaining primary cell cultures.
• MiniBrain (Organoid) Workshop – Explore 3D organoid techniques for modeling human brain development and disease.

Whether you are in academia or industry, our workshops provide practical skills, best practices, and technical insights that accelerate research and innovation in regenerative medicine and cell biology.

Human iPSC Culture Workshop

Accelerate your stem cell research with hands-on training in human induced pluripotent stem cell (hiPSC) cultureat the STEMTECH Center. This immersive workshop equips researchers with the knowledge and practical skills to independently culture, expand, and manipulate hiPSCs for both basic and translational research applications.

The program combines focused lectures with interactive laboratory sessions, providing a comprehensive introduction to modern hiPSC technologies.

Foundational hiPSC Culture Techniques

• Principles of human iPSC biology and morphology
• Thawing and recovery of hiPSC lines
• Routine maintenance under feeder‑dependent and feeder‑free conditions
• Manual, enzymatic, and non‑enzymatic passaging
• Preparation of feeder‑free substrates (Matrigel, Geltrex, CELLStart, Vitronectin)
• Preparation of standard hiPSC culture media
• Cryopreservation and banking of hiPSCs
• Mycoplasma testing and basic quality control
• Sterile technique and contamination prevention

Intermediate Techniques

• Expansion of hiPSCs for downstream applications
• Generation of cell pellets for RNA/DNA extraction
• Cell proliferation and viability assays
• Differentiation principles and lineage‑specific cues
• Introduction to 3D organoid culture workflows

Advanced hiPSC Techniques

• Isolation and reprogramming of iPSCs from PBMCs and fibroblasts
• Gene transfer and cell engineering methods
• CRISPR‑based genome editing
• Transfection and transduction systems (viral and non‑viral)
• Characterization and release testing of hiPSC lines
• Differentiation into NPCs, neurons, cardiomyocytes, hepatocytes, and other lineages
• Advanced organoid derivation, expansion, freezing, thawing, and characterization
• Suspension culture systems for large‑scale PSC expansion
• And more!

Why Attend:

• Gain confidence and independence in hiPSC workflows
• Master techniques used in cutting-edge stem cell biology and regenerative medicine
• Learn from STEMTECH Center experts in a small-group, hands-on environment.

To request the agenda and obtain additional information, pleasecontact the Center

Neural Differentiation Workshop: From PSCs to NPCs and Neurons

This hands-on workshop provides practical training in neural differentiation from pluripotent stem cells (PSCs), including the generation of Neural Progenitor Cells (NPCs) and Neurons using both stepwise differentiation and transcription factor–based forward programming approaches (NGN2).

Participants will learn essential laboratory techniques through a combination of lectures and guided lab sessions.

Key Skills and Techniques Covered:

• Generation of single-cell PSC suspensions for differentiation workflows
• Optimization of seeding densities for NGN2/TF-based forward programming
• Seeding of PSCs for NPC and NGN2-induced neuron differentiation using monolayer protocols
• Embryoid body (EB) formation and culture for neural progenitor generation
• Feeding, maintenance, and handling of EBs in AggreWell plates
• Collection and replating of EBs for downstream differentiation
• Rosette selection and replating during monolayer-based NPC derivation
• Morphological evaluation of neural cultures and differentiation progression
• SMAD inhibition-based NPC differentiation protocols
• Passaging and cryopreservation of neural progenitor cells (NPCs)
• Maintenance and handling of mature neuronal cultures
• Phenotypic evaluation and characterization of NPCs and differentiated neurons
• And more!

To request the agenda and obtain additional information, pleasecontact the Center

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Culturing Primary Cells from Human and Animal Tissues Workshop

This workshop is focused on the isolation, culture, and maintenance of primary cells derived from human tissues.

This workshop is designed for researchers, students, and laboratory personnel seeking practical experience in primary cell culture techniques used in modern biomedical research. Participants will learn essential methodologies while emphasizing sterility, reproducibility, and good laboratory practices.

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Participants will receive training in the following areas:

• Tissue Processing and Cell Isolation
  Enzymatic and mechanical dissociation methods for obtaining viable primary cells.
• Aseptic Technique and Biosafety
  Best practices for safe handling of primary cultures and maintaining sterile conditions.
• Culture Initiation and Maintenance
  Media preparation, plating densities, substrate selection, and environmental requirements.
• Monitoring Cell Health and Morphology
  Identifying healthy versus stressed cells, detecting contamination, and troubleshooting common issues.
• Passaging and Short-Term Expansion
  Gentle handling and expansion strategies to preserve phenotype and viability.
• Cryopreservation and Recovery
  Freezing protocols, thawing techniques, and methods to optimize cell viability.
• Ethical and Regulatory Considerations
  Guidelines for working with human- and animal-derived tissues.
• And more!

Who Should Attend our Stem Cells Workshops:

• Graduate students and postdoctoral researchers
• Laboratory technicians and research staff
• Investigators beginning work with primary cell cultures, stem cells bioreactors, organoids.
• Researchers seeking to improve cell culture reproducibility

Format

• Hands-on laboratory training combined with focused lectures and expert guidance from the STEMTECH Center staff.
• Limited spaces available to ensure personalized training.

Language

• Workshops, training, and technical consultation are also available in Spanish upon request.

Registration and additional information

• Please contact the Center!